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BP003568: Cynomolgus IGF1R/CD221 Protein with the C-His Tag
Source: The recombinant Cynomolgus IGF1R/CD221 Protein, (amino acids Glu31-Asn908) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Cynomolgus IGF1R/CD221 protein with the His tag at the C-terminus has a predicted MW of 80.59 kDa (alpha subunit) and 19.28 kDa (beta subunit). Due to glycosylation, the protein migrates to 48-58 kDa (beta subunit) and 100-115 kDa (alpha subunit) and 130-150 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Cynomolgus IGF1R protein with the C-His tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-IGF1R Antibody protein with the C-hFc tag with the EC50 of 5.7 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Cynomolgus IGF1R/CD221 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
Shipping: The recombinant Cynomolgus IGF1R/CD221 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.
Syd Labs provides the following IGFBP Protein :
Cynomolgus IGFBP2 Protein
Human IGFBP2 Protein
Mouse IGFBP2 Protein
Human IGFBP-3 Protein
Human IGFBP-3R Protein
Mouse IGFBP-3R Protein
Human IGFBP-7 Protein
Mouse IGFBP-7 Protein
Syd Labs provides the following IGF Protein :
Biotinylated Human IGF1R Protein
Biotinylated Human IGF2R Protein
Cynomolgus IGF1R Protein
Human IGF1R Protein
Human IGF2R Domain 1-3 Protein
Human IGF2R Domain 1-7 Protein
Human IGF2R Protein
Human IGFI Protein
Human IGFII Protein
Mouse IGF1R Protein
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